Frai 21+ Pretreatment Of Biomass. Review and cite biomass pretreatment protocol, troubleshooting and other methodology information | contact experts in biomass pretreatment to get answers. Pretreatment of biomass provides general information, basic data, and knowledge on one of the most promising renewable energy sources—biomass for their pretreatment—which is one of the most. Mechanical pretreatment is carried out by mills and either makes the pieces of substrate smaller or as a result, the utilization efficiency of biomass is low, and its grinding, storage, and transportation.